Course Details

DOT Urine Specimen Proficiency Mock Demonstrations

What’s Required for DOT Certification


To finalize your DOT urine collector qualification, you must complete:


Five (5) consecutive, error-free mock collections
For each device type used (e.g., split-specimen cups)
Observed in real time by a qualified trainer
Properly documented on Federal Custody and Control Forms (CCFs)


Failure to complete mock collections correctly can delay certification and limit your ability to perform DOT collections.


Required Mock Collection Scenarios (As required under 49 CFR Part 40.35)


Your five mock collections must include each of the following scenarios:

  1. Uneventful Collection – A standard, problem-free urine collection
  2. Insufficient Quantity – Donor cannot provide the required 45 mL
  3. Temperature Out of Range – Specimen is outside 32–38°C (90–100°F)
  4. Refusal or Failure to Sign/Initial – Donor refuses required CCF steps
  5. Adulteration or Tampering Attempt – Donor has something in mouth or attempts to alter the specimen

All five must be completed consecutively and without error.


ATA Provides a Qualified Monitoring DOT-Compliant


Each mock collection must be monitored by a qualified trainer who meets DOT requirements, including one or more of the following:


  • At least one year conducting DOT collections
  • At least one year training DOT collectors
  • Completion of a DOT Train-the-Trainer program

The trainer must certify in writing that all mock collections were performed correctly and error-free.


Flexible Monitoring Options

In-person observation
Live webcam monitoring (real-time interaction required)


Remote monitoring is fully acceptable as long as it meets DOT real-time supervision standards.


Proper Documentation Is Mandatory


  • Each mock collection must be recorded on a Federal Custody and Control Form (CCF)
  • All five must be consecutive and error-free
  • Documentation must be retained as proof of DOT qualification


Don’t Delay Your Certification

Mock collections are not optional—they are a DOT-required step to becoming a qualified urine specimen collector. Our structured mock demonstration program ensures you complete them correctly, efficiently, and without risk of rejection.


 Purchase your DOT Urine Collector Mock Demonstrations today and complete your certification with confidence.

Purchase DOT Urine Specimen Proficiency Mock Demonstrations Course

$199.99

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Related Courses

Non DOT Urine Collector Training

Our Urine Collector Training covers proper specimen collection per industry best practices, when performing urine collections for non-DOT drug tests. This course does not qualify you to perform DOT urine specimen collections

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Our TPA Training prepares individuals to manage testing programs for others. Learn to oversee compliance, manage vendors, and follow DOT and non-DOT rules for client testing program success.